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Wall Defective Bacterial Medium

[所屬分類:培養基配方] [發布時間:2021-10-12] [發布人:網站管理員2] [閱讀次數:] [返回]
Wall Defective Bacterial Medium

山東(dong)拓普生物(wu)工程有(you)限公司 培(pei)養基配(pei)方 //ljxinglong.com

Composition per liter:

Sucrose...................................................................................... 100.0g
Papaic digest of soybean meal .................................................... 20.0g
Agarose ....................................................................................... 10.0g
Yeast extract................................................................................ 10.0g
NaCl .............................................................................................. 5.0g
MgSO4·7H2O ................................................................................ 2.5g
Horse serum ...........................................................................200.0mL
Cholesterol solution .................................................................10.0mL
pH 7.8 ± 0.2 at 25°C
Cholesterol Solution:
Composition per 10.0mL:
Cholesterol .................................................................................. 0.04g
Ethanol (95% solution) ............................................................10.0mL
Preparation of Cholesterol Solution: Add cholesterol to 10.0mL
of 95% ethanol. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except cholesterol so
lution and horse serum, to distilled/deionized water and bring volume
to 790.0mL. Mix thoroughly. Adjust pH to 7.8. Add 10.0mL of choles
terol solution. Mix thoroughly. Gently heat and bring to boiling. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Asepti
cally add 200.0mL of sterile horse serum. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of cell wall-deficient bacteria, such as L
forms, that depend on osmotic stabilization.
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