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W Medium

[所屬分類:培養基配方] [發布時間:2021-10-12] [發布人:網站管理員2] [閱讀次數:] [返回]
W Medium

山東拓普(pu)生物(wu)工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Sulfur .......................................................................................... 10.0g
KH2PO4......................................................................................... 3.0g
MgSO4·7H2O ................................................................................ 0.5g
CaCl2·2H2O................................................................................. 0.25g
(NH4)2SO4..................................................................................... 0.2g
FeSO4·7H2O.............................................................................10.0mg
pH 3.0 ± 0.2 at 25°C
Preparation of Sulfur: Sterilize by steaming at 100°C for 60 min
on 3 consecutive days.
Preparation of Medium: Add components, except sulfur, to dis
tilled/deionized water and bring volume to 990.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0g
of sterile sulfur. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Use: For the cultivation of Thiobacillus thiooxidans.
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