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VY/4-SWS Agar (DSMZ Medium 958)

[所屬分類:培養基配方] [發布時間:2021-10-12] [發布人:網站管理員2] [閱讀次數:] [返回]
VY/4-SWS Agar
(DSMZ Medium 958)

山(shan)東拓普生物工(gong)程有(you)限公司 培養基配方 //ljxinglong.com

Composition per 1001.0mL:

NaCl............................................................................................ 20.0g
Agar ............................................................................................ 15.0g
Yeast cell paste (baker’s yeast, washed in
deionized water) wet weight .................................................. 2.5g
Seawater salts solution..................................................................1.0L
Vitamin B12 solution ..................................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Seawater Salts Solution:
Composition per liter:
MgSO4·7H2O................................................................................ 8.0g
CaCl2·2H2O .................................................................................. 1.0g
KCl................................................................................................ 0.5g
NaHCO3...................................................................................... 0.16g
KBr ............................................................................................. 0.08g
SrCl2·6H2O ................................................................................. 0.03g
H3BO3 ......................................................................................... 0.02g
Ferric citrate................................................................................ 0.01g
di-Na-ß-glycerophosphate .......................................................... 0.01g
Trace elements solution SL-4 ....................................................1.0mL
Trace Elements Solution SL-4:
Composition per liter:
EDTA............................................................................................ 0.5g
FeSO4·7H2O.................................................................................. 0.2g
Trace elements solution SL-6 ................................................100.0mL
Preparation of Trace Elements Solution SL-4: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution SL-6:
Composition per liter:
H3BO3 ........................................................................................... 0.3g
CoCl2·6H2O .................................................................................. 0.2g
ZnSO4·7H2O ................................................................................. 0.1g
MnCl2·4H2O ............................................................................... 0.03g
Na2MoO4·H2O ............................................................................ 0.03g
NiCl2·6H2O................................................................................. 0.02g
CuCl2··2H2O................................................................................ 0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Preparation of Seawater Salts Solution: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin B12 Solution:
Composition per 10.0mL:
Cyanocobalamin ........................................................................ 5.0mg
Preparation of Vitamin B12 Solution: Add cyanocobalamine to
distilled/deionized water and bring volume to 10.0mL. Mix thorough
ly. Sparge with 100% N2. Filter sterilize.
Preparation of Medium: Add NaCl, agar, and yeast cell paste to
1.0L seawater salts solution. Mix thoroughly. Adjust pH to 7.5 with 1M
NaOH. Gently heat and bring to boiling. Autoclave for 20 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add 1.0mL sterile vitamin
B12 solution. Mix thoroughly. Pour into sterile Petri dishes or leave in
tubes.
Use: For the cultivation and maintenance of Haliangium ochraceum,
Haliangium tepidum, Plesiocystis pacifica, and Enhygromyxa salina
(Thaxtera salina).
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