培養基配方

您現在的位置：網站首頁 >> 培養基配方

Universal Beer Agar

[所屬分類:培養基配方] [發布時間:2021-12-24] [發布人:網站管理員2] [閱讀次數:] [返回]

Universal Beer Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Peptonized milk ................................................15.0g

Agar ..................................................................12.0g

Yeast extract......................................................10.0g

Glucose .............................................................10.0g

Tomato juice solids .............................................7.0g

K2HPO4...............................................................0.5g

KH2PO4...............................................................0.5g

MgSO4·5H2O ......................................................0.2g

NaCl..................................................................0.01g

FeSO4·7H2O......................................................0.01g

MnSO4·H2O ......................................................0.01g

Beer............................................................. 250.0mL

pH 6.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components, ex

cept beer, to distilled/deionized water and bring vol

ume to 750.0mL. Mix thoroughly. Gently heat and

bring to boiling. Add beer. Mix thoroughly. Distrib

ute into tubes or flasks. Autoclave for 10 min at 15

psi pressure–121°C. Cool to 50°C. Aseptically add

250.0mL of beer without degassing. Pour into sterile

Petri dishes or leave in tubes.

Use: For the enumeration of contaminating bacteria

and yeasts encountered in wort and beer.