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Tween 80 Hydrolysis Broth

[所屬分類:培養基配方] [發布時間:2021-12-24] [發布人:網站管理員2] [閱讀次數:] [返回]
Tween 80 Hydrolysis Broth

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per 125.0mL:

Neutral Red.........................................................0.1g
Solution 1...................................................... 38.9mL
Solution 2...................................................... 61.1mL
Tween 80....................................................... 25.0mL
pH 7.0 ± 0.2 at 25°C
Solution 1:
Composition per 400.0mL:
KH2PO4.............................................................22.7g
Preparation of Solution 1: Add KH2PO4 to dis
tilled/deionized water and bring volume to 400.0mL.
Mix thoroughly.
Solution 2:
Composition per 400.0mL:
Na2HPO4 ...........................................................23.8g
Preparation of Solution 2: Add Na2HPO4 to dis
tilled/deionized water and bring volume to 400.0mL.
Mix thoroughly.
Preparation of Medium: Combine components.
Mix thoroughly. Distribute into tubes or flasks. Auto
clave for 15 min at 15 psi pressure–121°C.
Use: For the differentiation of Mycobacterium spe
cies. Strains that hydrolyze Tween 80 within 5 days
turn the medium pink to red.

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