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培養基配方

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TSN Agar (Trypticase Sulfite Neomycin Agar)

[所屬分類:培養基配方] [發布時間:2021-12-24] [發布人:網站管理員2] [閱讀次數:] [返回]

TSN Agar

(Trypticase Sulfite Neomycin Agar)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................15.0g

Agar ..................................................................13.5g

Yeast extract......................................................10.0g

Na2SO3................................................................1.0g

Ferric citrate........................................................0.5g

Neomycin sulfate ..............................................0.05g

Polymyxin sulfate .............................................0.02g

Buffered thioglycolate solution..................... 50.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Buffered Thioglycolate Solution:

Composition per 50.0mL:

Buffer solution .............................................. 35.0mL

Sodium thioglycolate solution ...................... 15.0mL

Preparation of Buffered Thioglycolate Solu

tion: Combine components. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C.

Buffer Solution:

Composition per 100.0mL:

Na2CO3 .............................................................28.0g

K2HPO4...............................................................5.7g

Preparation of Buffer Solution: Add compo

nents to distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly.

Thioglycolate Solution:

Composition per 100.0mL:

Sodium thioglycolate........................................13.3g

Preparation of Thioglycolate Solution: Add

sodium thioglycolate to distilled/deionized water and

bring volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Add components, ex

cept buffered thioglycolate solution, to distilled/

deionized water and bring volume to 950.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 12 min at 13 psi pressure–118°C. Do not

overheat. Cool to 45°–50°C. Aseptically add buff

ered thioglycolate solution. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation of Clostridium per

fringens.

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TSN Agar (Trypticase Sulfite Neomycin Agar)