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Tryptone Phosphate Brain Heart Infusion Yeast Extract Agar (TPBY)

[所屬分類:培養基配方] [發布時間:2021-12-23] [發布人:網站管理員2] [閱讀次數:] [返回]
Tryptone Phosphate Brain Heart
Infusion Yeast Extract Agar
(TPBY)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................20.0g
Agar ..................................................................15.0g
NaCl..................................................................5.14g
K2HPO4...............................................................2.0g
KH2PO4...............................................................2.0g
Yeast extract........................................................1.0g
Oxgall..................................................................0.5g
Pancreatic digest of gelatin.................................0.4g
Brain heart, solids from infusion ......................0.16g
Peptic digest of animal tissue............................0.16g
Glucose .............................................................0.08g
Na2HPO4 ...........................................................0.06g
Tween 80......................................................... 1.5mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Adjust pH to 7.0. Distribute into tubes or flasks. Au
toclave for 15 min at 15 psi pressure–121°C. Pour
into sterile Petri dishes or leave in tubes.
Use: For the cultivation of coliform bacteria, such as
Escherichia coli, from foods.
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