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Trypticase Peptone Glucose Yeast Extract Broth with Trypsin (TPGYT Broth) (BAM M151a)

[所屬分類:培養基配方] [發布時間:2021-12-22] [發布人:網站管理員2] [閱讀次數:] [返回]

Trypticase Peptone Glucose

Yeast Extract Broth with Trypsin

(TPGYT Broth)

(BAM M151a)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 1067.0mL:

Pancreatic digest of casein................................50.0g

Yeast extract......................................................20.0g

Peptone ...............................................................5.0g

Glucose ...............................................................4.0g

Sodium thioglycolate..........................................1.0g

Trypsin solution ............................................ 67.0mL

pH 7.0 ± 0.2 at 25°C

Trypsin Solution:

Composition per 100.0mL:

Trypsin ................................................................1.5g

Preparation of Trypsin Solution: Add trypsin

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept trypsin solution, to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Gently heat

and bring to boiling. Distribute into tubes in 15.0mL

volumes. Autoclave for 10 min at 15 psi pressure–

121°C. Immediately prior to use, aseptically add

1.0mL of sterile trypsin solution to each tube. Mix

thoroughly.

Use: For the cultivation of Clostridium botulinum.