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Tryptic Soy Fast Green Agar (TSFA)

[所屬分類:培養基配方] [發布時間:2021-12-22] [發布人:網站管理員2] [閱讀次數:] [返回]
Tryptic Soy Fast Green Agar
(TSFA)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................17.0g
Agar ..................................................................15.0g
NaCl....................................................................5.0g
Papaic digest of soybean meal............................3.0g
K2HPO4...............................................................2.5g
Glucose ...............................................................2.5g
Fast Green FCF.................................................0.25g
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti
cally adjust pH to 7.3. Pour into sterile Petri dishes.
Use: For the isolation and cultivation of Salmonella
species from foods.
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