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山東拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Antibiotic HiVeg Assay Medium No. 5
(Streptomycin HiVeg Agar with Yeast Extract)
(Antibiotic HiVeg Assay Medium - E)
Composition per liter:
Agar ............................................................................................15.0g
Plant peptone.................................................................................6.0g
Yeast extract..................................................................................3.0g
Plant extract..................................................................................1.5g
pH 7.9 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For antibiotic assay testing. For the streptomycin assay using the
cylinder plate technique and Bacillus subtilis as test organism.