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Thioglycolate Medium without Glucose and Indicator

[所屬分類:培養基配方] [發布時間:2021-12-21] [發布人:網站管理員2] [閱讀次數:] [返回]
Thioglycolate Medium
without Glucose and Indicator

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................15.0g
Yeast extract........................................................5.0g
NaCl....................................................................2.5g
Agar ..................................................................0.75g
L-Cystine ...........................................................0.25g
Thioglycollic acid ........................................... 0.3mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. If medium becomes oxi
dized before use, heat in a boiling water bath to expel
absorbed O2. Cool to 25°C.
Use: For the cultivation of anaerobic, microaero
philic, and aerobic microorganisms. For use in steril
ity testing of a variety of specimens.
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