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TCH Medium (Thiophene 2 Carboxylic Acid Hydrazide Medium)

[所屬分類:培養基配方] [發布時間:2021-12-20] [發布人:網站管理員2] [閱讀次數:] [返回]
TCH Medium
(Thiophene 2 Carboxylic
Acid Hydrazide Medium)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per 1105.0mL:

Thiophene-2-carboxylic acid hydrazide ..........1.1mg
Middlebrook 7H10 agar base............................. 1.0L
OADC enrichment...................................... 100.0mL
Glycerol .......................................................... 5.0mL
pH 6.6 ± 0.2 at 25°C
Middlebrook 7H10 Agar Base:
Composition per liter:
Agar ..................................................................15.0g
Na2HPO4.............................................................1.5g
KH2PO4...............................................................1.5g
(NH4)2SO4 ..........................................................0.5g
L-Glutamic acid...................................................0.5g
Sodium citrate.....................................................0.4g
Ferric ammonium citrate...................................0.04g
MgSO4·7H2O..................................................0.025g
ZnSO4·7H2O ....................................................1.0mg
CuSO4·5H2O....................................................1.0mg
Pyridoxine........................................................1.0mg
Biotin ...............................................................0.5mg
CaCl2·2H2O......................................................0.5mg
Malachite Green.............................................0.25mg
Preparation of Middlebrook 7H10 Agar
Base: Add glycerol to 900.0mL of distilled/deion
ized water and add remaining components. Mix thor
oughly. Gently heat and bring to boiling.
Middlebrook OADC Enrichment:
Composition per 100.0mL:
Bovine albumin fraction V .................................5.0g
Glucose ...............................................................2.0g
NaCl..................................................................0.85g
Oleic acid ..........................................................0.05g
Catalase............................................................4.0mg
Source: This enrichment is available as a prepared
enrichment from BD Diagnostic Systems.
Preparation of Middlebrook OADC Enrich
ment: Add components to distilled/deionized water
and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation for Medium: Combine compo
nents. Mix thoroughly. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C. Pour
into sterile Petri dishes or leave in tubes.
Use: For the differentiation of Mycobacterium spe
cies based on sensitivity to TCH. Mycobacterium bo
vis is inhibited by TCH. Mycobacterium tuberculosis
and other mycobacteria are generally resistant to low
concentrations of TCH. This distinguishes Mycobac
terium bovis from other nonchromogenic, slow
growing mycobacteria.
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