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Tarshis Blood Agar

[所屬分類:培養基配方] [發布時間:2021-12-20] [發布人:網站管理員2] [閱讀次數:] [返回]
Tarshis Blood Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per 1050.0mL:

Beef heart infusion..........................................500.0g
Agar ..................................................................15.0g
Meat peptone ....................................................10.0g
NaCl....................................................................5.0g
Penicillin G, sterile.....................................100,000U
Sheep blood, sterile..................................... 300.0mL
Glycerol ........................................................ 10.0mL
pH 6.6 ± 0.2 at 25°C
Preparation of Medium: Add components, ex
cept sheep blood and penicillin G, to distilled/deion
ized water and bring volume to 750.0mL. Mix
thoroughly. Gently heat and bring to boiling. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add sterile sheep blood and
sterile penicillin G. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Mycobacte
rium tuberculosis.
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