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Sucrose Yeast Extract Medium

[所屬分類:培養基配方] [發布時間:2021-12-20] [發布人:網站管理員2] [閱讀次數:] [返回]
Sucrose Yeast Extract Medium

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Sucrose..............................................................20.0g
Yeast extract........................................................4.0g
K2HPO4...............................................................2.5g
MgSO4·7H2O ......................................................1.0g
Trace elements solution .................................. 4.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
ZnSO4·7H2O .....................................................18.0g
FeSO4·7H2O........................................................9.0g
MnSO4·4H2O......................................................3.0g
CoCl2·6H2O ........................................................0.9g
CuSO4·5H2O.......................................................0.8g
H2SO4 (Concentrated) .................................... 5.0mL
Preparation of Trace Elements Solution: Add
components to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of a variety of bacteria that
can utilize sucrose as carbon source.
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