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Streptococcus Selective Medium

[所屬分類:培養基配方] [發布時間:2021-12-17] [發布人:網站管理員2] [閱讀次數:] [返回]
Streptococcus Selective Medium

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Special peptone.................................................23.0g
Agar ..................................................................10.0g
NaCl....................................................................5.0g
Starch ..................................................................1.0g
Horse blood, defibrinated ............................. 50.0mL
Antibiotic inhibitor ....................................... 10.0mL
pH 7.3± 0.2 at 25°C
Source: This medium is available as a premixed
powder from Oxoid.
Antibiotic Inhibitor:
Composition per 10.0mL:
Colistin sulfate...............................................10.0mg
Oxolinic acid....................................................5.0mg
Preparation of Antibiotic Inhibitor: Add com
ponents to distilled/deionized water and bring vol
ume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept horse blood and antibiotic inhibitor, to distilled/
deionized water and bring volume to 940.0mL. Mix
thoroughly. Gently heat and bring to boiling. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add sterile horse blood and
sterile antibiotic inhibitor. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the selective isolation of streptococci from
clinical specimens or foodstuffs.
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