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Streptococcal Growth Medium

[所屬分類:培養基配方] [發布時間:2021-12-17] [發布人:網站管理員2] [閱讀次數:] [返回]
Streptococcal Growth Medium

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Beef heart, solids from infusion......................500.0g
Tryptose ............................................................10.0g
NaCl....................................................................5.0g
Glucose ...............................................................1.0g
Bromcresol Purple solution ............................ 1.0mL
pH 7.4 ± 0.2 at 25°C
Bromcresol Purple Solution:
Composition per 10.0mL:
Bromcresol Purple ............................................0.16g
Ethanol (95% solution) ................................. 10.0mL
Preparation of Bromcresol Purple Solution:
Add Bromcresol Purple to 10.0mL of ethanol. Mix
thoroughly.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Distribute into tubes in 5.0mL vol
umes. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of Streptococcus species and
other Gram-positive cocci. Growth in this medium
turns the indicator yellow and the solution turbid.
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