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Starch Hydrolysis Agar

[所屬分類:培養基配方] [發布時間:2021-12-17] [發布人:網站管理員2] [閱讀次數:] [返回]
Starch Hydrolysis Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Beef heart, infusion from................................500.0g
Soluble starch....................................................20.0g
Agar ..................................................................15.0g
Tryptose ............................................................10.0g
NaCl....................................................................5.0g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dish
es or leave in tubes.
Use: For the cultivation and differentiation of a vari
ety of microorganisms based on amylase production.
After incubation, starch hydrolysis is determined by
the addition of Gram’s or Lugol’s iodine solution. Or
ganisms that produce amylase appear as colonies sur
rounded by a clear zone.
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