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Standard Methods Caseinate Agar

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Standard Methods Caseinate Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g
Sodium caseinate ..............................................10.0g
Pancreatic digest of casein..................................5.0g
Yeast extract........................................................2.5g
Glucose ...............................................................1.0g
Trisodium citrate solution.................................. 1.0L
CaCl2·2H2O (1M solution)............................ 20.0mL
Trisodium Citrate Solution:
Composition per liter:
Trisodium citrate·2H2O.....................................4.41g
Preparation of Trisodium Citrate Solution:
Add trisodium citrate·2H2O to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add yeast extract, pan
creatic digest of casein, glucose, and agar to 500.0mL
of the trisodium citrate solution. Mix thoroughly. Gen
tly heat until boiling. Add the remaining components.
Bring volume to 1.0L with trisodium citrate solution.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Pour into sterile Petri dishes.
Use: For the cultivation of proteolytic bacteria from
foods.
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