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Spray’s Fermentation Medium (BAM M141)

[所屬分類:培養基配方] [發布時間:2021-12-17] [發布人:網站管理員2] [閱讀次數:] [返回]
Spray’s Fermentation Medium
(BAM M141)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per 1100.0mL:

Neopeptone .......................................................10.0g
Pancreatic digest of casein................................10.0g
Agar ....................................................................2.0g
Sodium thioglycolate ......................................0.025g
Carbohydrate solution................................. 110.0mL
pH 7.4 ± 0.1 at 25°C
Carbohydrate Solution:
Composition per 200.0mL:
Carbohydrate.....................................................20.0g
Preparation of Carbohydrate Solution: Add
carbohydrate to distilled/deionized water and bring
volume to 200.0mL. Glucose or glycerol may be
used. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept agar and carbohydrate solution, to distilled/
deionized water and bring volume to 990.0mL. Mix
thoroughly. Adjust pH to 7.4. Add agar. Gently heat
and bring to boiling. Distribute into tubes in 9.0mL
volumes. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 25°C. Immediately prior to use, heat
tubes in a boiling water bath for 10 min. Cool to
45°C. Aseptically add 1.0mL of sterile carbohydrate
solution. Mix thoroughly.
Use: For the cultivation and differentiation of
Clostridium perfringens based on carbohydrate fer
mentation patterns.
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