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Sorbitol MacConkey Agar (MacConkey Agar with Sorbitol) (BAM M139)

[所屬分類:培養基配方] [發布時間:2021-12-16] [發布人:網站管理員2] [閱讀次數:] [返回]
Sorbitol MacConkey Agar
(MacConkey Agar with Sorbitol)
(BAM M139)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Peptone or gelysate...........................................17.0g
Agar ..................................................................13.5g
Sorbitol .............................................................10.0g
NaCl....................................................................5.0g
Proteose peptone No. 3 or polypeptone..............3.0g
Bile salts, purified...............................................1.5g
Neutral Red.......................................................0.03g
Crystal Violet...................................................1.0mg
pH 7.1 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dish
es or leave in tubes.
Use: For the isolation and cultivation of pathogenic
Escherichia coli.
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