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Sorbitol Agar

[所屬分類:培養基配方] [發布時間:2021-12-16] [發布人:網站管理員2] [閱讀次數:] [返回]
Sorbitol Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................20.0g
Peptone..............................................................10.0g
NaCl....................................................................2.0g
Yeast extract........................................................2.0g
Sorbitol solution............................................ 50.0mL
pH 7.0 ± 0.2 at 25°C
Sorbitol Solution:
Composition per 50.0mL:
Sorbitol................................................................5.0g
Preparation of Sorbitol Solution: Add sorbitol
to distilled/deionized water and bring volume to
50.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept sorbitol solution, to distilled/deionized water
and bring volume to 950.0mL. Mix thoroughly. Ad
just pH to 7.0. Gently heat and bring to boiling. Au
toclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add sterile sorbitol solution.
Mix thoroughly. Pour into sterile Petri dishes or dis
tribute into sterile tubes.
Use: For the cultivation and maintenance of
Pseudomonas species.
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