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Slad Medium

[所屬分類:培養基配方] [發布時間:2021-12-16] [發布人:網站管理員2] [閱讀次數:] [返回]
Slad Medium

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Solution B ................................................... 900.0mL
Solution A................................................... 100.0mL
Solution A:
Composition per 100.0mL:
Raffinose...........................................................20.0g
KH2PO4...............................................................1.0g
MgSO4·7H2O ......................................................0.5g
NaCl....................................................................0.1g
CaCl2·2H2O.........................................................0.1g
Ammonium sulfate ..........................................7.0mg
Inositol .............................................................2.0mg
KI .....................................................................1.0mg
H3BO3 ..............................................................0.5mg
ZnSO4·7H2O ....................................................0.4mg
MnSO4·4H2O ...................................................0.4mg
Thiamine·HCl ..................................................0.4mg
Pyroxidine·HCl ................................................0.4mg
Niacin...............................................................0.4mg
Calcium pantothenate ......................................0.4mg
p-Aminobenzoic acid.......................................0.2mg
Riboflavin ........................................................0.2mg
FeCl3 ................................................................0.2mg
Na2MoO4·4H2O ...............................................0.2mg
CuSO4·5H2O..................................................0.04mg
Folic acid ..........................................................2.0μg
Biotin ................................................................2.0μg
Preparation of Solution A: Add components to
distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Filter sterilize. Warm to
50°–55°C.
Solution B:
Composition per 900.0mL:
Agar ..................................................................40.0g
Preparation of Solution B: Add agar to distilled/
deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to
50°–55°C.
Preparation of Medium: Aseptically combine
100.0mL of sterile solution A and 900.0mL of sterile
solution B. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use: For the cultivation of Saccharomyces cerevisiae.
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