培養基配方

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Skirrow Brucella Medium

[所屬分類:培養基配方] [發布時間:2021-12-16] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Blood agar base No. 2................................. 940.0mL

Horse blood, lysed defibrinated.................... 50.0mL

Antibiotic solution ........................................ 10.0mL

pH 7.4 ± 0.2 at 25°C

Blood Agar Base No. 2

Composition per 940.0mL:

Proteose peptone...............................................15.0g

Agar ..................................................................12.0g

NaCl....................................................................5.0g

Yeast extract........................................................5.0g

Liver digest .........................................................2.5g

pH 7.4 ± 0.2 at 25°C

Preparation of Blood Agar Base No. 2: Add

components to distilled/deionized water and bring

volume to 940.0mL. Mix thoroughly. Gently heat

while stirring and bring to boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Antibiotic Solution:

Composition per 10.0mL:

Vancomycin ......................................................0.01g

Trimethoprim...................................................5.0mg

Polymyxin B ...................................................2500U

Preparation of Antibiotic Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 940.0mL of sterile

cooled blood agar base No. 2, aseptically add 50.0mL

of sterile, lysed defibrinated horse blood and 10.0mL

of sterile antibiotic solution. Pour into sterile Petri

dishes or distribute into sterile tubes.

Use: For the selective isolation and cultivation of

Campylobacter species.