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Single-Layer Agar

[所屬分類:培養基配方] [發布時間:2021-12-16] [發布人:網站管理員2] [閱讀次數:] [返回]
Single-Layer Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per 1050.0mL:

Basal medium .................................................... 1.0L
Tributyrin substrate...........................................50.0g
pH 6.8 ± 0.2 at 25°C
Basal Medium:
Composition per liter:
Agar ..................................................................15.0g
Pancreatic digest of gelatin.................................5.0g
Beef extract.........................................................3.0g
Preparation of Basal Medium: Add agar to
1.0L of distilled/deionized water. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°C.
Tributyrin Substrate:
Composition:
Tributyrin substrate...........................................50.0g
Preparation of Tributyrin Substrate: Remove
free fatty acids in the tributyrin substrate by dissolv
ing 50.0g in 500.0mL of petroleum ether. Pass the so
lution through an activated alumina column. Remove
the petroleum ether by evaporation on a steam table
under 100% N2. Autoclave for 30 min at 15 psi pres
sure–121°C. Cool to 50°C.
Preparation of Medium: Aseptically combine
1.0L of sterile basal medium with 50.0g of sterile
tributyrin substrate in a warm, sterile blender con
tainer. Blend for 1 min until homogenized. Rapidly
pour into sterile Petri dishes in 7.0mL volumes. Dry
the surface of the plates by partially opening the lids
in a laminar flow hood for 15 min.
Use: For the isolation, cultivation, and identification
of lipolytic microorganisms from food.
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