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Seven H11 Agar (Selective 7H11 Agar)

[所屬分類:培養基配方] [發布時間:2021-12-16] [發布人:網站管理員2] [閱讀次數:] [返回]
Seven H11 Agar
(Selective 7H11 Agar)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per 1010.0mL:

Agar ..................................................................13.5g
KH2PO4...............................................................1.5g
Na2HPO4 .............................................................1.5g
Pancreatic digest of casein..................................1.0g
NaCl..................................................................0.85g
Monosodium glutamate ......................................0.5g
(NH4)2SO4...........................................................0.5g
Sodium citrate.....................................................0.4g
MgSO4·7H2O ....................................................0.05g
Ferric ammonium citrate...................................0.04g
CuSO4·5H2O ....................................................1.0mg
Pyridoxine........................................................1.0mg
ZnSO4·7H2O ....................................................1.0mg
Biotin ...............................................................0.5mg
CaCl2·2H2O......................................................0.5mg
Malachite Green.............................................0.25mg
Glycerol .......................................................... 5.0mL
Middlebrook OADC enrichment ................ 100.0mL
Antibiotic inhibitor ....................................... 10.0mL
pH 6.6 ± 0.2 at 25°C
Source: This medium is available as a prepared me
dium from BD Diagnostic Systems.
Middlebrook OADC Enrichment:
Bovine albumin fraction V .................................5.0g
Glucose ...............................................................2.0g
NaCl..................................................................0.85g
Catalase............................................................3.0mg
Oleic acid ...................................................... 0.06mL
Preparation of Middlebrook OADC Enrich
ment: Add components to distilled/deionized water
and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Antibiotic Inhibitor:
Composition per 10.0mL:
Carbenicillin......................................................0.05g
Trimethoprim lactate.........................................0.02g
Amphotericin B ................................................0.01g
Polymyxin B ..............................................200,000U
Preparation of Antibiotic Inhibitor: Add com
ponents to distilled/deionized water and bring vol
ume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add glycerol to
900.0mL of distilled/deionized water. Mix thorough
ly. Add remaining components, except Middlebrook
OADC enrichment and antibiotic inhibitor. Mix thor
oughly. Gently heat. Do not boil. Autoclave for 10
min at 15 psi pressure–121°C. Cool to 50°–55°C.
Aseptically add 100.0mL of sterile Middlebrook
OADC enrichment and 10.0mL of sterile antibiotic
solution. Mix thoroughly. Pour into sterile Petri dish
es or distribute into sterile tubes.
Use: For the isolation and cultivation of Mycobacte
rium species from specimens with a mixed flora.
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