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培養基配方

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Serum Glucose Agar, Farrell Modified

[所屬分類:培養基配方] [發布時間:2021-12-16] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 1086.9mL:

Agar ..................................................................15.0g

Peptone..............................................................10.0g

Beef extract.........................................................5.0g

NaCl....................................................................5.0g

Serum-glucose solution................................. 60.0mL

Bacitracin solution ........................................ 12.5mL

Cycloheximide solution ................................ 10.0mL

Nystatin solution............................................. 2.0mL

Polymyxin B solution ..................................... 1.0mL

Nalidixic acid solution.................................... 1.0mL

Vancomycin solution....................................... 0.4mL

pH 7.3 ± 0.2 at 25°C

Serum-Glucose Solution:

Composition per 60.0mL:

D-Glucose ..........................................................10.0g

Serum (inactivated at 56°C, 30 min) ............ 50.0mL

Preparation of Serum-Glucose Solution: Add

glucose to 50.0mL of heat-inactivated serum. Horse

serum or ox serum may be used. Mix thoroughly. Fil

ter sterilize.

Bacitracin Solution:

Composition per 12.5mL:

Bacitracin.....................................................25,000U

Preparation of Bacitracin Solution: Add Baci

tracin to distilled/deionized water and bring volume

to 12.5mL. Mix thoroughly. Filter sterilize.

Cycloheximide Solution:

Composition per 100.0mL:

Cycloheximide....................................................1.0g

Acetone ........................................................... 5.0mL

Preparation of Cycloheximide Solution: Add

cycloheximide to 5.0mL of acetone. Mix thoroughly.

Bring volume to 100.0mL with distilled/deionized

water. Mix thoroughly. Filter sterilize.

Nystatin Solution:

Composition per 5.0mL:

Nystatin......................................................250,000U

Preparation of Nystatin Solution: Add nystatin

to distilled/deionized water and bring volume to

5.0mL. Mix thoroughly. Filter sterilize.

Polymyxin B Solution:

Composition per 2.0mL:

Polymyxin B ................................................10,000U

Preparation of Polymyxin B Solution: Add

polymyxin B to distilled/deionized water and bring

volume to 2.0mL. Mix thoroughly. Filter sterilize.

Nalidixic Acid Solution:

Composition per 2.0mL:

Nalidixic acid......................................................0.1g

NaOH (0.5N solution)..................................... 2.0mL

Preparation of Nalidixic Acid Solution: Add

nalidixic acid to 2.0mL of NaOH solution. Mix thor

oughly. Immediately before use, add 1.0mL of this

stock solution to 9.0mL of distilled/deionized water.

Mix thoroughly. Filter sterilize.

Vancomycin Solution:

Composition per 1.0mL:

Vancomycin ......................................................0.05g

Preparation of Vancomycin Solution: Add

vancomycin to distilled/deionized water and bring

volume to 1.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components—ex

cept serum-glucose solution, bacitracin solution, cy

cloheximide solution, nystatin solution, polymyxin B

solution, nalidixic acid solution, and vancomycin so

lution—to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 10 psi pressure–

115°C. Cool to 50°C. Aseptically add 60.0mL of

sterile serum-glucose solution, 12.5mL of sterile ba

citracin solution, 10.0mL of sterile cycloheximide

solution, 2.0mL of sterile nystatin solution, 1.0mL of

sterile polymyxin B solution, 1.0mL of sterile nalid

ixic acid solution, and 0.4mL of sterile vancomycin

solution. Mix thoroughly. Pour into sterile Petri dish

es or distribute into sterile tubes. Allow tubes to cool

in a slanted position.

Use: For the selective isolation and cultivation of

Brucella species.

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Serum Glucose Agar, Farrell Modified