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Semisolid Medium for Motility

[所屬分類:培養基配方] [發布時間:2021-12-16] [發布人:網站管理員2] [閱讀次數:] [返回]
Semisolid Medium for Motility

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Biosate ................................................................5.0g
Polypeptone ........................................................5.0g
NaCl....................................................................5.0g
Agar ....................................................................4.0g
Myosate...............................................................1.5g
Triphenyltetrazolium chloride solution .......... 2.5mL
pH 6.9–7.1at 25°C
Triphenyltetrazolium Chloride Solution:
Composition per 10.0mL:
Triphenyltetrazolium chloride ............................0.1g
Ethanol (95% solution)................................. 10.0mL
Preparation of Triphenyltetrazolium Chlo
ride Solution: Add triphenyltetrazolium chloride
to 10.0mL of ethanol. Mix thoroughly.
Preparation of Medium: Add components, ex
cept triphenyltetrazolium chloride solution, to dis
tilled/deionized water and bring volume to 997.5mL.
Mix thoroughly. Gently heat and bring to boiling.
Add 2.5mL of triphenyltetrazolium chloride solu
tion. Mix thoroughly. Distribute into tubes in 10.0mL
volumes. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the differentiation of bacteria based on mo
tility.
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