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培養基配方
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Selection Agar II
[所屬分類:培養基配方] [發布時間:2021-12-15] [發布人:網站管理員2] [閱讀次數:] [返回]
Selection Agar II
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Sodium acetate..................................................40.0g
Agar ..................................................................25.0g
(NH4)2SO4 ..........................................................5.0g
Maltose ...............................................................2.5g
KH2PO4...............................................................1.0g
MgSO4·7H2O......................................................0.5g
NaCl....................................................................0.1g
CaCl2·2H2O ........................................................0.1g
Inositol .............................................................2.0mg
KI .....................................................................1.0mg
H3BO3 ..............................................................0.5mg
ZnSO4·7H2O ....................................................0.4mg
MnSO4·4H2O...................................................0.4mg
Thiamine·HCl ..................................................0.4mg
Pyroxidine·HCl ................................................0.4mg
Niacin...............................................................0.4mg
Calcium pantothenate ......................................0.4mg
p-Aminobenzoic acid.......................................0.2mg
Riboflavin ........................................................0.2mg
FeCl3 ................................................................0.2mg
Na2MoO4·4H2O ...............................................0.2mg
CuSO4·5H2O..................................................0.04mg
Folic acid ..........................................................2.0μg
Biotin ................................................................2.0μg
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dish
es or leave in tubes.
Use: For the selective cultivation of Saccharomyces
cerevisiae.
