- 定做培養基/定制培養基
- 顆粒培養基
- 標準菌株生化鑒定試劑盒
- 預灌裝即用型成品培養基
- 2025年版中國藥典
- 促銷/特價商品
- 院感/疾控/體外診斷/采樣管
- 樣品采集與處理(均質)產品
- 按標準檢索培養基
- 模擬灌裝用培養基
- 干燥粉末培養基
- 培養基添加劑/補充劑
- 生化反應鑒定管
- 染色液等配套產品
- 對照培養基/標準品
- 實驗耗材與器具
- 生化試劑/化學試劑
- 菌種鑒定服務
培養基配方
您現在的位置: 網站首頁 >> 培養基配方
Schleifer-Krämer Agar (SK Agar)
[所屬分類:培養基配方] [發布時間:2021-12-15] [發布人:網站管理員2] [閱讀次數:] [返回]
Schleifer-Krämer Agar
(SK Agar)
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Agar ..................................................................13.0g
Glycerol ............................................................10.0g
Sodium pyruvate...............................................10.0g
Pancreatic digest of casein................................10.0g
Beef extract.........................................................5.0g
Yeast extract........................................................3.0g
Potassium isothiocyanate..................................2.25g
LiCl .....................................................................2.0g
Na2HPO4·2H2O...................................................0.9g
NaH2PO4·H2O.....................................................0.6g
Glycine................................................................0.5g
NaN3 solution................................................ 10.0mL
pH 7.2 ± 0.2 at 25°C
NaN3 Solution:
Composition per 10.0mL:
NaN3 ...............................................................0.045g
Preparation of NaN3 Solution: Add NaN3 to
distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept NaN3 solution, to distilled/deionized water and
bring volume to 990.0mL. Mix thoroughly. Adjust
pH to 7.2. Gently heat and bring to boiling. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add sterile NaN3 solution.
Mix thoroughly. Pour into sterile Petri dishes or dis
tribute into sterile tubes.
Use: For the isolation and cultivation of Staphylo
coccus species.



