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SC Agar

[所屬分類:培養基配方] [發布時間:2021-12-15] [發布人:網站管理員2] [閱讀次數:] [返回]
SC Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................20.0g
(NH4)2SO4...........................................................5.0g
KH2PO4...............................................................1.0g
MgSO4·7H2O ......................................................0.5g
NaCl....................................................................0.1g
CaCl2·2H2O.........................................................0.1g
Inositol .............................................................2.0mg
KI .....................................................................1.0mg
H3BO3 ..............................................................0.5mg
ZnSO4·7H2O ....................................................0.4mg
MnSO4·4H2O ...................................................0.4mg
Thiamine·HCl ..................................................0.4mg
Pyroxidine·HCl ................................................0.4mg
Niacin...............................................................0.4mg
Calcium pantothenate ......................................0.4mg
p-Aminobenzoic acid.......................................0.2mg
Riboflavin ........................................................0.2mg
FeCl3 ................................................................0.2mg
Na2MoO4·4H2O ...............................................0.2mg
CuSO4·5H2O ..................................................0.04mg
Folic acid.......................................................... 2.0μg
Biotin ............................................................... 2.0μg
Glucose solution ........................................... 50.0mL
Synthetic stock solution................................ 30.0mL
Glucose Solution:
Composition per 100.0mL:
Glucose .............................................................40.0g
Preparation of Glucose Solution: Add glucose
to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Filter sterilize.
Synthetic Stock Solution:
Composition per 300.0mL:
L-Isoleucine.......................................................5.25g
L-Arginine.........................................................3.48g
L-Aspartic acid..................................................2.66g
L-Leucine ..........................................................2.62g
L-Methionine.....................................................1.49g
L-Threonine.......................................................1.19g
L-Valine.............................................................1.17g
L-Serine.............................................................1.05g
L-Lysine ............................................................0.91g
L-Phenylalanine ................................................0.83g
L-Tryptophan ....................................................0.82g
L-Histidine ........................................................0.58g
m-Inositol..........................................................0.36g
Uracil ................................................................0.22g
L-Tyrosine .........................................................0.18g
Adenine...........................................................0.135g
Preparation of Synthetic Stock Solution: Add
components to distilled/deionized water and bring
volume to 300.0mL. Mix thoroughly. Filter sterilize.
Store in dark at 25°C.
Preparation of Medium: Add components, ex
cept synthetic stock solution and glucose solution, to
distilled/deionized water and bring volume to
920.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°–55°C. Aseptically add 30.0mL
of sterile synthetic stock solution and 50.0mL of ster
ile glucose solution. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Saccha
romyces cerevisiae.
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