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培養基配方

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Saccharolytic Clostridia Medium

[所屬分類:培養基配方] [發布時間:2021-12-14] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................10.0g

Yeast extract........................................................6.0g

Sodium thioglycolate ..........................................0.5g

Carbohydrate solution................................. 100.0mL

Potassium phosphate

(1M solution, pH 7.5) ............................. 30.0mL

MgSO4 (1M solution) ..................................... 1.0mL

Solution M ...................................................... 0.5mL

FeSO4 solution ................................................ 0.2mL

pH 7.0–7.2 at 25°C

Carbohydrate Solution:

Composition per 100.0mL:

Glucose or sucrose............................................20.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Solution M:

Composition per liter:

CaCl2.................................................................3.33g

MnCl2·4H2O .....................................................1.98g

Na2MoO4·2H2O ................................................1.21g

CoCl2·6H2O ......................................................1.19g

Preparation of Solution M: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly.

FeSO4·7H2O Solution:

Composition per 10.0mL:

FeSO4·7H2O......................................................55.6g

H2SO4 (0.1M solution).................................. 10.0mL

Preparation of FeSO4·7H2O Solution: Add the

FeSO4·7H2O to 10.0mL of H2SO4 solution. Mix

thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 900.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 20

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile carbohydrate solution. Mix

thoroughly. Aseptically distribute into sterile tubes.

Use: For the cultivation of saccharolytic Clostridium

species.

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Saccharolytic Clostridia Medium