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培養基配方

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SA Agar

[所屬分類:培養基配方] [發布時間:2021-12-14] [發布人:網站管理員2] [閱讀次數:] [返回]

SA Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of casein................................10.0g

NaCl....................................................................5.0g

Starch, soluble.....................................................1.0g

Ampicillin.........................................................0.01g

Phenol Red......................................................0.018g

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components, ex

cept ampicillin, to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add

ampicillin. Mix thoroughly. Pour into sterile Petri

dishes.

Use: For the isolation, cultivation, and differentiation,

based on starch hydrolysis, of Aeromonas hydrophila

from foods. After inoculation of plates and growth of

cultures, starch hydrolysis is determined by flooding

each plate with 5.0mL of Lugol’s iodine solution.

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