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培養基配方

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Rogosa SL Broth (Rogosa Selective Lactobacillus Broth)

[所屬分類:培養基配方] [發布時間:2021-12-14] [發布人:網站管理員2] [閱讀次數:] [返回]

Rogosa SL Broth

(Rogosa Selective Lactobacillus Broth)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Sodium acetate..................................................15.0g

Glucose .............................................................10.0g

Pancreatic digest of casein................................10.0g

K2HPO4...............................................................6.0g

Yeast extract........................................................5.0g

Arabinose............................................................5.0g

Sucrose................................................................5.0g

Ammonium citrate..............................................2.0g

Sorbitan monooleate ...........................................1.0g

MgSO4·7H2O....................................................0.57g

MnSO4·7H2O....................................................0.12g

FeSO4·H2O........................................................0.03g

Acetic acid, glacial........................................ 1.32mL

pH 5.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components, ex

cept glacial acetic acid, to distilled/deionized water

and bring volume to 998.7mL. Mix thoroughly. Gen

tly heat and bring to boiling. Add glacial acetic acid.

Mix thoroughly. Gently heat while stirring and bring

to 90°–100°C for 2–3 min. Do not autoclave. Asepti

cally distribute into sterile tubes.

Use: For the isolation, cultivation, and enumeration

of lactobacilli, especially from dairy products.

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