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Riboflavin Assay Medium

[所屬分類:培養基配方] [發布時間:2021-12-14] [發布人:網站管理員2] [閱讀次數:] [返回]

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Composition per liter:

Peptone, photolyzed..........................................22.0g

Glucose .............................................................20.0g

Yeast supplement ................................................2.0g

Sodium acetate....................................................1.8g

K2HPO4...............................................................1.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O......................................................0.4g

L-Cystine .............................................................0.2g

FeSO4·7H2O...................................................20.0mg

MnSO4·H2O...................................................20.0mg

NaCl...............................................................20.0mg

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes in 5.0mL vol

umes. Add standard solutions and test solutions to

each tube. Bring volume of each tube to 10.0mL. Au

toclave for 10 min at 15 psi pressure–121°C.

Use: For the microbiological assaying of riboflavin

using Lactobacillus casei as the test organism.