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培養基配方

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RB-1/RB-9 Medium

[所屬分類:培養基配方] [發布時間:2021-12-14] [發布人:網站管理員2] [閱讀次數:] [返回]

RB-1/RB-9 Medium

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Glucose or starch ..............................................10.0g

Na2 HPO4·12H2O................................................4.2g

KH2PO4.............................................................2.64g

Yeast extract........................................................2.0g

Na2SO4................................................................1.0g

NH4Cl .................................................................0.5g

L-Cysteine·HCl ...................................................0.5g

Na2S·9H2O..........................................................0.5g

MgCl2·6H2O .....................................................0.36g

Resazurin .........................................................1.0mg

Trace elements solution ................................ 10.0mL

Vitamin solution............................................ 10.0mL

pH 5.0–6.0 at 25°C

Vitamin Solution:

Composition per liter:

Pyridoxine·HCl ..............................................10.0mg

Calcium DL-pantothenate.................................5.0mg

Lipoic acid .......................................................5.0mg

Nicotinic acid...................................................5.0mg

p-Aminobenzoic acid.......................................5.0mg

Riboflavin ........................................................5.0mg

Thiamine·HCl ..................................................5.0mg

Biotin ...............................................................2.0mg

Folic acid .........................................................2.0mg

Vitamin B12 ......................................................0.1mg

Preparation of Vitamin Solution: Add compo

nents to distilled/deionized water and bring volume

to 1.0L. Mix thoroughly. Sparge with 100% N2. Au

toclave for 15 min at 15 psi pressure–121°C.

Trace Elements Solution:

Composition per liter:

MgSO4·7H2O ..................................................... 3.0g

Nitrilotriacetic acid .............................................1.5g

NaCl....................................................................1.0g

MnSO4·2H2O ..................................................... 0.5g

CoSO4·7H2O .....................................................0.18g

ZnSO4·7H2O .....................................................0.18g

CaCl2·2H2O.........................................................0.1g

FeSO4·7H2O........................................................0.1g

NiCl2·6H2O .....................................................0.025g

KAI(SO4)2·12H2O.............................................0.02g

CuSO4·5H2O .....................................................0.01g

H3BO3 ...............................................................0.01g

Na2MoO4·2H2O ............................................... 0.01g

Na2SeO3·5H2O.................................................0.3mg

Preparation of Trace Elements Solution: Add

nitrilotriacetic acid to 500.0mL of distilled/deionized

water. Adjust pH to 6.5 with KOH. Add remaining

components. Add distilled/deionized water to 1.0L.

Preparation of Medium: Prepare and dispense

medium under 100% N2. Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Sparge with 100% N2. Adjust pH to 5.0–

6.0. Anaerobically distribute into tubes or flasks. Au

toclave for 20 min at 15 psi pressure–121°C.

Use: For the cultivation of Clostridium species.

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RB-1/RB-9 Medium