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Rappaport Broth, Modified (Rap Broth, Modified)

[所屬分類:培養基配方] [發布時間:2021-12-14] [發布人:網站管理員2] [閱讀次數:] [返回]
Rappaport Broth, Modified
(Rap Broth, Modified)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per 250.2mL:

Solution A................................................... 155.0mL
Solution C ..................................................... 53.0mL
Solution B ..................................................... 40.0mL
Solution D....................................................... 1.6mL
Solution E ....................................................... 0.6mL
Solution A:
Composition per liter:
Pancreatic digest of casein................................10.0g
Preparation of Solution A: Add pancreatic di
gest of casein to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly.
Solution B:
Composition per liter:
Na2HPO4.............................................................9.5g
Preparation of Solution B: Add Na2HPO4 to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly.
Solution C:
Composition per 100.0mL:
MgCl2·6H2O .....................................................40.0g
Preparation of Solution C: Add MgCl2·6H2O to
distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 25°C.
Solution D:
Composition per 100.0mL:
Malachite Green..................................................0.2g
Preparation of Solution D: Add Malachite
Green to sterile distilled/deionized water and bring
volume to 100.0mL. Mix thoroughly. Do not steril
ize.
Solution E:
Composition per 10.0mL:
Carbenicillin .....................................................0.01g
Preparation of Solution E: Add carbenicillin to
distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Combine 155.0mL of
solution A and 40.0mL of solution B. Mix thorough
ly. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C. Aseptically add 53.0mL of sterile
solution C, 1.6mL of solution D, and 0.6mL of sterile
solution E. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the isolation and cultivation of Yersinia en
terocolitica from foods.
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