培養基配方

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Purple Agar

[所屬分類:培養基配方] [發布時間:2021-12-13] [發布人:網站管理員2] [閱讀次數:] [返回]

Purple Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g

Proteose peptone No. 3 .....................................10.0g

NaCl....................................................................5.0g

Beef extract.........................................................1.0g

Bromcresol Purple ............................................0.02g

Carbohydrate solution................................... 20.0mL

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Carbohydrate Solution:

Composition per 20.0mL:

Carbohydrate.....................................................10.0g

Preparation of Carbohydrate Solution: Add

carbohydrate to distilled/deionized water and bring

volume to 20.0mL. For expensive carbohydrates,

5.0g may be used instead of 10.0g. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, ex

cept carbohydrate solution, to distilled/deionized wa

ter and bring volume to 980.0mL. Mix thoroughly.

Gently heat and bring to boiling. Distribute into tubes

in 9.8mL volumes. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add

0.2mL of sterile carbohydrate solution to each tube.

Mix thoroughly. Allow tubes to cool in a slanted po

sition.

Use: For the preparation of carbohydrate media used

in fermentation studies for the identification of bac

teria, especially members of the Enterobacteriaceae.

Bacteria that can ferment the carbohydrate turn the

medium yellow.