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培養基配方

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Pseudomonas aeruginosa Agar (PA Agar) (m–PA Agar) (m-Pseudomonas aeruginosa Agar)

[所屬分類:培養基配方] [發布時間:2021-12-13] [發布人:網站管理員2] [閱讀次數:] [返回]

Pseudomonas aeruginosa Agar

(PA Agar)

(m–PA Agar)

(m-Pseudomonas aeruginosa Agar)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g

Na2S2O3 ..............................................................6.8g

L-Lysine·HCl.......................................................5.0g

NaCl....................................................................5.0g

Xylose.................................................................2.5g

Yeast extract........................................................2.0g

Lactose..............................................................1.25g

Sucrose..............................................................1.25g

Ferric ammonium citrate.....................................0.8g

Sulfapyridine...................................................0.176g

Cycloheximide..................................................0.15g

Phenol Red........................................................0.08g

Nalidixic acid..................................................0.037g

Kanamycin.......................................................8.5mg

pH 7.1 ± 0.2 at 25°C

Preparation of Medium: Add components—ex

cept sulfapyridine, cycloheximide, nalidixic acid,

and kanamycin—to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Adjust pH to

6.5. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 55°–60°C. Readjust pH to 7.1. Aseptically

add the sulfapyridine, cycloheximide, nalidixic acid,

and kanamycin. Mix thoroughly. Pour into 50mm ×

12mm Petri dishes in 3.0mL volumes.

Use: For the cultivation and estimation of numbers

of Pseudomonas aeruginosa by the membrane filter

method.