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培養基配方

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Powell and Errington’s Medium

[所屬分類:培養基配方] [發布時間:2021-12-10] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 1060.0mL:

Solution 1...................................................... 50.0mL

Solution 3...................................................... 50.0mL

Solution 4...................................................... 10.0mL

Solution 2........................................................ 5.0mL

pH 7.0 ± 0.2 at 25°C

Solution 1:

Composition per liter:

(NH4)2HPO4....................................................238.0g

K2SO4 ...............................................................70.0g

NaH2PO4·2H2O.................................................31.0g

Preparation of Solution 1: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly.

Solution 2:

Composition per liter:

MgO..................................................................10.0g

FeCl3·6H2O.........................................................5.4g

CaCO3 .................................................................2.0g

ZnSO4·7H2O .....................................................1.44g

MnSO4·4H2O....................................................1.11g

Na2MoO4·2H2O ................................................0.49g

CoSO4·7H2O.....................................................0.28g

CuSO4·5H2O.....................................................0.25g

H3BO4 .............................................................0.062g

HCl, concentrated ......................................... 50.0mL

Preparation of Solution 2: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly.

Solution 3:

Composition per 50.0mL:

Citric acid............................................................4.2g

Glucose ...............................................................3.6g

L-Glutamic acid.................................................2.94g

Succinic acid.....................................................1.18g

Preparation of Solution 3: Add components to

distilled/deionized water and bring volume to

50.0mL. Mix thoroughly. Filter sterilize.

Solution 4:

Composition per 10.0mL:

Na2S2O3·5H2O ..................................................1.24g

Preparation of Solution 4: Add Na2S2O3·5H2O

to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add 50.0mL of solu

tion 1 and 5.0mL of solution 2. Mix thoroughly.

Bring volume to 1.0L with distilled/deionized water.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 25°C. Adjust pH to 7.0 with sterile NaOH. Asepti

cally add 50.0mL of solution 3 and 10.0mL of sterile

solution 4. Mix thoroughly. Aseptically distribute

into sterile tubes or flasks.

Use: For the cultivation of a variety of heterotrophic

microorganisms.

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Powell and Errington’s Medium