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Potato Dextrose Salt Agar (BAM M127)

[所屬分類:培養基配方] [發布時間:2021-12-10] [發布人:網站管理員2] [閱讀次數:] [返回]
Potato Dextrose Salt Agar
(BAM M127)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

NaCl..................................................................75.0g
Agar ..................................................................20.0g
Glucose .............................................................20.0g
Potato infusion................................................... 1.0L
pH 5.6 ± 0.2 at 25°C
Potato Infusion:
Composition per 1.0L:
Potatoes, unpeeled and sliced .........................200.0g
Preparation of Potato Infusion: Add unpeeled
potato slices to 1.0L of distilled/deionized water.
Gently heat and bring to boiling. Continue boiling for
30 min. Filter through cheesecloth. Reserve filtrate.
Bring volume to 1.0L with distilled/deionized water.
Preparation of Medium: Add agar and glucose
to 1.0L potato infusion. Mix thoroughly. Gently heat
and bring to boiling. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C. Pour
into sterile Petri dishes or leave in tubes.
Use: For the cultivation and enumeration of holo
philic yeasts and halophilic filamentous fungi
(molds) from foods.
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