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Potato Dextrose Broth with Yeast Extract

[所屬分類:培養基配方] [發布時間:2021-12-10] [發布人:網站管理員2] [閱讀次數:] [返回]
Potato Dextrose Broth
with Yeast Extract

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Potato, peeled and cut .....................................200.0g
Glucose .............................................................10.0g
Yeast extract........................................................3.0g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Peel and cut potatoes.
Add potatoes to 500.0mL of water. Boil potatoes for
20 min. Filter through cheesecloth. Add glucose and
yeast extract to filtrate. Bring volume to 1.0L with
distilled/deionized water. Mix thorougly. Adjust pH
to 7.2. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C.
Use: For the cultivation of a variety of fungi.
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