<dd id='n3mmm'></dd>

培養基配方

您現在的位置：網站首頁 >> 培養基配方

Potato Dextrose Agar (PDA Agar) (ATCC Medium 336)

[所屬分類:培養基配方] [發布時間:2021-12-10] [發布人:網站管理員2] [閱讀次數:] [返回]

Potato Dextrose Agar

(PDA Agar)

(ATCC Medium 336)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Glucose .............................................................20.0g

Agar ..................................................................15.0g

Potatoes, infusion from............................... 500.0mL

pH 5.6 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Potato Infusion:

Composition per 500.0mL:

Potatoes...........................................................300.0g

Preparation of Potato Infusion: Peel and dice

potatoes. Add 500.0mL of distilled/deionized water.

Gently heat and bring to boiling. Continue boiling for

30 min. Filter through cheesecloth. Reserve filtrate.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the cultivation of yeasts and molds from

dairy products and other foods. Also used to induce

sporulation in many fungi.