培養基配方

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Potato Dextrose Agar

[所屬分類:培養基配方] [發布時間:2021-12-10] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Glucose .............................................................20.0g

Agar ..................................................................15.0g

Potato, infusion from ..........................................4.0g

Tartaric acid solution .................................... 14.0mL

pH 5.6 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Tartaric Acid Solution:

Composition per 50.0mL:

Tartaric acid ........................................................5.0g

Preparation of Tartaric Acid Solution: Add

tartaric acid to distilled/deionized water and bring

volume to 50.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 986.0mL.

Mix thoroughly. Gently heat and bring to boiling. Dis

tribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically add

14.0mL of sterile tartaric acid solution. Mix thoroughly.

If medium is to be used for the enumeration of yeasts

and molds in butter, adjust pH to 3.5. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the cultivation and enumeration of yeasts

and molds. For the enumeration of yeasts and molds

in butter by the plate count method.