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Potato Agar with Cereal Culms

[所屬分類:培養基配方] [發布時間:2021-12-10] [發布人:網站管理員2] [閱讀次數:] [返回]
Potato Agar with Cereal Culms

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Potatoes...........................................................200.0g
Agar ..................................................................20.0g
Cereal culms .................................................variable
Preparation of Medium: Peel and dice potatoes.
Add potatoes to 1.0L of tap water. Gently heat and
bring to boiling. Boil potatoes until thoroughly
cooked. Filter through cheesecloth. Bring volume of
filtrate to 1.0L with tap water. Add 20.0g of agar.
Gently heat and bring to boiling. Distribute into
tubes. Autoclave for 15 min at 15 psi pressure–
121°C. Cut cereal culms into 1.0cm pieces. Place 6 to
9 cereal culm pieces in tubes with 3.0mL of distilled/
deionized water. Autoclave for 15 min at 15 psi pres
sure–121°C. Melt agar tubes by gently heating. Cool
to 50°C. Aseptically add 2 to 3 sterile cereal culm
pieces to each tube. Allow to solidify in a slanted po
sition.
Use: For the cultivation of fungi.
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