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Potassium Cyanide Broth (BAM M126)

[所屬分類:培養基配方] [發布時間:2021-12-10] [發布人:網站管理員2] [閱讀次數:] [返回]
Potassium Cyanide Broth
(BAM M126)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Na2HPO4...........................................................5.64g
NaCl....................................................................5.0g
Proteose peptone No. 3.......................................3.0g
KH2PO4...........................................................0.225g
KCN solution................................................ 15.0mL
pH 7.6 ± 0.2 at 25°C
KCN Solution:
Composition per 100.0mL:
KCN....................................................................0.5g
Preparation of KCN Solution: Add KCN to dis
tilled/deionized water and bring volume to 100.0mL.
Mix thoroughly.
Caution: Cyanide is toxic. Do not pipet by mouth.
Use protective equipment/gloves when handling.
Preparation of Medium: Add components, ex
cept KCN solution, to distilled/deionized water and
bring volume to 985.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 25°C. Aseptically add
15.0mL of KCN solution. Mix thoroughly. Distribute
into sterile screw-capped tubes or flasks in 1.0–
1.5mL volumes. Close caps tightly. Store at 5–8°C no
longer than 2 weeks before use.
Use: For the cultivation and differentiation of ure
ase-negative, Gram-negative enteric bacteria. Salmo
nella species and Shigella species are nonmotile in
this medium. Proteus species are motile in this medi
um.
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