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Polypectate Gel Medium

[所屬分類:培養基配方] [發布時間:2021-12-9] [發布人:網站管理員2] [閱讀次數:] [返回]
Polypectate Gel Medium

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Sodium polypectate ..........................................70.0g
K2HPO4...............................................................5.0g
Peptone ...............................................................5.0g
KH2PO4...............................................................1.0g
CaCl2·2H2O ........................................................0.6g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except
sodium polypectate, to 500.0mL of boiling water. Put
into a blender and mix on low speed to dissolve. Add the
sodium polypectate slowly with the blender on low
speed to minimize air bubbles. Adjust pH to 7.0. Bring
volume to 1.0L with distilled/deionized water. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Pour into sterile Petri dishes.
Use: For the cultivation of microoganisms and de
tection of pectate-degrading enzymes.
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