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培養基配方
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PM Indicator Agar
[所屬分類:培養基配方] [發布時間:2021-12-9] [發布人:網站管理員2] [閱讀次數:] [返回]
PM Indicator Agar
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Agar ..................................................................15.0g
Glucose .............................................................5.25g
Peptone................................................................5.0g
Beef extract.........................................................3.0g
Pancreatic digest of casein..................................1.7g
Sorbitan monooleate complex ............................1.0g
NaCl....................................................................0.5g
Papaic digest of soybean meal............................0.3g
K2HPO4.............................................................0.25g
Bromcresol Purple ............................................0.06g
pH 7.8 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling. Do
not autoclave. Inoculate medium with a suspension
of Bacillus stearothermophilus spores. Pour into
100mm flat-bottomed Petri dishes in 6.0mL vol
umes. Use medium immediately.
Use: For the rapid detection of trace amounts of pen
icillin in milk using the AOAC Bacillus stearother
mophilus Qualitative Disc Method II.



