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Phenol Red Mannitol Broth

[所屬分類:培養基配方] [發布時間:2021-12-9] [發布人:網站管理員2] [閱讀次數:] [返回]
Phenol Red Mannitol Broth

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................10.0g
D-Mannitol..........................................................5.0g
NaCl....................................................................5.0g
Phenol Red......................................................0.018g
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Adjust pH to 7.3 if necessary. Dis
tribute into tubes containing an inverted Durham
tube. Fill each tube with 10.0mL of medium. Auto
clave for 15 min at 13 psi pressure–118°C.
Use: For determination of the ability of a microor
ganism to ferment mannitol. Fermentation is deter
mined by the production of acid—broth turns
yellow—and formation of gas—bubble trapped in
Durham tube.
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