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Phenol Red Glucose Broth with NaCl (BAM M122)

[所屬分類:培養基配方] [發布時間:2021-12-9] [發布人:網站管理員2] [閱讀次數:] [返回]

Phenol Red Glucose Broth with NaCl
(BAM M122)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

NaCl..................................................................30.0g
Proteose peptone No. 3.....................................10.0g
Glucose ...............................................................5.0g
Beef extract.........................................................1.0g
Phenol Red solution........................................ 7.2mL
pH 7.4 ± 0.2 at 25°C
Source: This medium without added NaCl is avail
able as a premixed powder from BD Diagnostics.
Phenol Red Solution:
Composition per 10.0mL:
Phenol Red......................................................0.025g
Preparation of Phenol Red Solution: Add Phe
nol Red to distilled/deionized water and bring vol
ume to 10.0mL. Mix thoroughly.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Adjust pH to 7.4 if necessary. Dis
tribute into tubes containing an inverted Durham
tube. Fill each tube with 10.0mL of medium. Auto
clave for 10 min at 13 psi pressure–118°C.
Use: For determination of the ability of halophilic
Vibrio spp. to ferment glucose. Fermentation is deter
mined by the production of acid—broth turns yel
low—and formation of gas—bubble trapped in
Durham tube.

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