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Phenethyl Alcohol Agar (Phenylethanol Agar) (Phenylethyl Alcohol Agar)

[所屬分類:培養基配方] [發布時間:2021-12-9] [發布人:網站管理員2] [閱讀次數:] [返回]

Phenethyl Alcohol Agar

(Phenylethanol Agar)

(Phenylethyl Alcohol Agar)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of casein................................15.0g

NaCl....................................................................5.0g

Papaic digest of soybean meal............................5.0g

β-Phenethyl alcohol............................................2.5g

Blood............................................................. 50.0mL

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components, ex

cept blood, to distilled/deionized water and bring

volume to 950.0mL. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 13 psi

pressure–118°C. Cool to 45°–50°C. Aseptically add

sterile defibrinated blood. Mix thoroughly. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation of Gram-positive

bacteria, particularly Gram-positive cocci, from

specimens with a mixed flora. Do not use for the ob

servation of hemolytic reactions.

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